Content

In this practical couse studens perform DNA isolation and purification, restriction enzyme digests, agarose gel electrophoresis, PCR an QPCR transformation of plasmid vectors in host bacteria and heterologous protein expression. Working with genetically modified micro-organisms demands specific aseptic handling techniques and laboratory organization. Special attention is paid on this topic and on working with small volumes using automated micropipettes. The interpretations and use of English standard protocols is taught, as well as the procedures of scientific record keeping. This course also illustrates the theoretical course Gene technology- basic techniques (2BLt). Finally, this course offers an initiation to laboratory skills applied in the next year (for 3FBLt).

Introduction
Safety, sterilisation and waste mangement 
Pipetting of small volumes using automatic pipettes 
Agarosegel electroforesis
Restriction enzym digests
PCR- Alu repeat en analysis
Plasmid vectors– identification of recombinants
Transformation of plasmids and induced  gene expression
QPCR
 

Course material

Study cost: 1-10 euros (The information about the study costs as stated here gives an indication and only represents the costs for purchasing new materials. There might be some electronic or second-hand copies available as well. You can use LIMO to check whether the textbook is available in the library. Any potential printing costs and optional course material are not included in this price.)

Lydia Hendriks (2014),  Lab gene technology 1, non published course, Geel 

additional:
 
Sambrook, J., Fritsch, E.F. and T. Maniatis ( 1989).  Molecular Cloning, A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press, CSH-USA. 
Brown, T.A. (2010), Gene cloning and DNA analysis, an introduction, Chapman and Hall, London, 5th ed 

Format: more information

Realization: The student train practical skills during labsessions. Students prepare  each lab session: students actively participate and work together and communicate in the total group during the practical lessons.  Students keep a lab notebook.
Concrete learning: The student performs different basic DNA analysis and transformation experiments.
Timing: the sessions are organized per half day (3 hours). Timing is communicated via Toledo.

Organization and supervision: The teacher can be consistently consuted during the lab sessions for advice and feedbackThis course explains and illustrates basic skills in DNA technology.

Content

 The student will apprehend how the knowledge of naturally occurring DNA processes (see Molecular biology) can be applied to basic DNA manipulation techniques. The student will also get insight in more complex and/or highly automated biomedical applications and analyses.

Isolation  of nucleic acids and DNA 
Restriction enzymes 
Gel electrophoresis 
Cloning of DNA fragments into plasmid vectors: amplification and expression
PCR : polymerase chain reaction- the basics

QPCR

DNA sequence analyses
Hybridisation techniques 
Molecular diagnostics 
     RFLP
     ASO/SSO

    ligation assays

    expression microarray

Course material

Study cost: 1-10 euros (The information about the study costs as stated here gives an indication and only represents the costs for purchasing new materials. There might be some electronic or second-hand copies available as well. You can use LIMO to check whether the textbook is available in the library. Any potential printing costs and optional course material are not included in this price.)

Hendriks, L. (2011) non published course Gene technology 1 -basic techniques 
Presentations and extra info on Toledo 

 Brown, T.A. (2006) Gene cloning and DNA analysis, an introduction, Chapman and Hall, London, 5th ed .

Format: more information

Realization: The course is designed so that students get information during a contact time. During these moments also exercises are made ( 'Molecular Diagnostics' ). The student performs a small number of excersises outside the lesson. These tasks are described in the course: eg consulting electronic databases such as eg restriction enzyme databases, etc.

Concrete learning:
The student can independently translate the information obtained during the course into the exercises. He is able to independently  comprehend the basis DNA technology

Timing: The contact moments are weekly, unless otherwise communicated.
Organization & guidance: The student makes individual assignments. During the contact sessions, the theoretical background is given; afterwards, the student will work independently. During this contact sessions, the student can ask for additional information to the lecturer.